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Frontiers
frontiersin.org › journals › bioengineering-and-biotechnology › articles › 10.3389 › fbioe.2023.1149588 › full
Frontiers | Antimicrobial, anticancer, and biofilm inhibition studies of highly reduced graphene oxide (HRG): In vitro and in silico analysis
February 23, 2023 - Both ampicillin and gentamicin inhibit MRSA biofilm with higher concentrations of 200 μg/mL and 250 μg/mL in comparison to HRG, and concentration inverted microscopic results are included in Figure 9. Graphene and materials made from it (GMs) showed a variety of antibacterial activities against viruses, fungi, and bacteria (Alangari et al., 2022). The primary source of these effects is thought to be the direct physicochemical contact between GMs and bacteria, resulting in the fatal destruction of biological components, primarily proteins, lipids, and nucleic acids (Mohammed et al., 2020).
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Frontiers
frontiersin.org › journals › microbiology › articles › 10.3389 › fmicb.2021.623853 › full
Frontiers | Graphene-Based Nanomaterials Modulate Internal Biofilm Interactions and Microbial Diversity
February 28, 2021 - Here and according to the obtained data, we suggest that the biofilm responses were mainly associated to indirect effects initiated by the overproduction of EPS by the diatom. This potentially increased the amount of carbon available for bacterial metabolism and further benefit to the diatom. However, the oxidized form of graphene was shown to strongly impact bacterial biomass.
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PubMed Central
pmc.ncbi.nlm.nih.gov › articles › PMC10304026
Effects of Graphene Oxide Nanosheets in Freshwater Biofilms - PMC
A significant increase in the enzymatic (SOD and GSTs activity) and low molecular weight (lipids and carotenoids) antioxidant response was observed, that efficiently reduced oxidative stress effects, reducing the level of peroxidation, and preserving membrane integrity. Being complex entities, biofilms are more similar to environmental communities and may provide more accurate information to evaluate the impact of GO in aquatic systems. Keywords: graphene oxide, freshwater biofilms, shading, photosynthetic pigments, oxidative damage, antioxidant activity
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PubMed
pubmed.ncbi.nlm.nih.gov › 31250387
Antibacterial effect of graphene oxide (GO) nano-particles against Pseudomonas putida biofilm of variable age - PubMed
Graphene oxide (GO) has been reported to possess antibacterial activity; therefore, its accumulation in the environment could affect microbial communities such as biofilms. The susceptibility of biofilms to antimicrobials is known to depend ...
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ScienceDirect
sciencedirect.com › science › article › abs › pii › S0010938X25003154
Indirect impact of quorum sensing on corrosion of graphene-coated copper via sulfate-reducing bacteria biofilm enhancement - ScienceDirect
April 30, 2025 - Graphene is widely explored as a non-invasive coating to prevent corrosion by microbial biofilms, yet its vulnerability to quorum sensing (QS)-regulated colonization remains unclear.
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ACS Publications
pubs.acs.org › doi › 10.1021 › acsomega.7b00371
Graphene Oxide-Coated Surface: Inhibition of Bacterial Biofilm Formation due to Specific Surface–Interface Interactions | ACS Omega
The graphene surface provides an effective barrier thereby preventing physical contact between the bacteria and underlying substrate to substantially suppress interaction, which is an essential requirement for the establishment of the biofilm.
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PubMed Central
pmc.ncbi.nlm.nih.gov › articles › PMC11433944
Impact of Graphene Layers on Genetic Expression and Regulation within Sulfate-Reducing Biofilms - PMC
According to recent studies, graphene promotes the formation of biofilms and bacterial growth [13,14,15]. Graphene is a promising candidate for biological, antifouling, and anticorrosion applications despite the lack of substantial research in these areas. A small number of papers have reported ...
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Springer
link.springer.com › home › journal of materials science › article
Recent advances and mechanism of antimicrobial efficacy of graphene-based materials: a review | Journal of Materials Science | Springer Nature Link
May 8, 2023 - Fallatah H, Elhaneid M, Ali-Boucetta H et al (2019) Antibacterial effect of graphene oxide (GO) nano-particles against Pseudomonas putida biofilm of variable age. Environ Sci Pollut Res 26:25057–25070.
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PubMed
pubmed.ncbi.nlm.nih.gov › 36770342
Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments - PubMed
January 18, 2023 - Biofilm architecture analysis indicated that mature biofilms developed on the graphene-based surface had fewer empty spaces (34% reduction) and reduced biovolume (25% reduction) compared to PDMS.
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PubMed Central
pmc.ncbi.nlm.nih.gov › articles › PMC10071309
Antimicrobial, anticancer, and biofilm inhibition studies of highly reduced graphene oxide (HRG): In vitro and in silico analysis - PMC
Both ampicillin and gentamicin inhibit MRSA biofilm with higher concentrations of 200 μg/mL and 250 μg/mL in comparison to HRG, and concentration inverted microscopic results are included in Figure 9. Graphene and materials made from it (GMs) showed a variety of antibacterial activities against viruses, fungi, and bacteria (Alangari et al., 2022). The primary source of these effects is thought to be the direct physicochemical contact between GMs and bacteria, resulting in the fatal destruction of biological components, primarily proteins, lipids, and nucleic acids (Mohammed et al., 2020).
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MDPI
mdpi.com › 2079-4991 › 13 › 3 › 381
Graphene-Based Coating to Mitigate Biofilm Development in Marine Environments
January 18, 2023 - For the last four incubation weeks, ... results indicate that the graphene-based polymeric coating showed significant antibacterial and antibiofilm performance....
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PubMed Central
pmc.ncbi.nlm.nih.gov › articles › PMC7701186
Graphene: An Antibacterial Agent or a Promoter of Bacterial Proliferation? - PMC
On the one hand, multiple reports describe GMs promoting bacterial growth and biofilm formation (ElMekawy et al., 2017; Ruiz et al., 2011; Hegab et al., 2016). In these studies, pristine graphene (Gr), graphene oxide (GO), and reduced graphene oxide (rGO) are put forward as high-performance materials for diverse applications where bacterial metabolism must be stimulated such as power generation by microbial fuel cells (MFCs) or the acceleration of methane production by anaerobic digestion (Cotts et al., 2020; Tian et al., 2017).
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PubMed
pubmed.ncbi.nlm.nih.gov › 30679015
Hydrophobicity of graphene as a driving force for inhibiting biofilm formation of pathogenic bacteria and fungi - PubMed
TiGD presented high quality and coverage and decreased biofilm formation for all species. The increased hydrophobicity of graphene films was correlated with the decreased biofilm formation for various species.
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BMC Veterinary Research
bmcvetres.biomedcentral.com › articles › 10.1186 › s12917-022-03560-6
Antimicrobial activities of graphene oxide against biofilm and intracellular Staphylococcus aureus isolated from bovine mastitis | BMC Veterinary Research | Full Text
January 14, 2023 - Here the study demonstrated graphene oxide antimicrobial efficacy against biofilm and intracellular S. aureus isolated from subclinical cases mastitis cases in Malaysia. GO was effective against the extracellular, intracellular, and biofilms forms of S. aureus. The intracellular antimicrobial activities appeared to be dependent on the actin polymerization of the membrane cell that controls the macropinocytosis uptake pathway of the cells.
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ACS Publications
pubs.acs.org › doi › 10.1021 › acs.estlett.5b00066
Interaction of Graphene Oxide with Bacterial Cell Membranes: Insights from Force Spectroscopy | Environmental Science & Technology Letters
The graphene oxide nanowalls were obtained by electrophoretic deposition of Mg2+-graphene oxide nanosheets synthesized by a chem. exfoliation method. On the basis of measuring the efflux of cytoplasmic materials of the bacteria, it was found that the cell membrane damage of the bacteria caused by direct contact of the bacteria with the extremely sharp edges of the nanowalls was the effective mechanism in the bacterial inactivation.
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PubMed
pubmed.ncbi.nlm.nih.gov › 29143262
Influences of graphene oxide on biofilm formation of gram-negative and gram-positive bacteria - PubMed
In this study, we evaluated the influences of graphene oxide (GO) on biofilm formation. Escherichia coli MG1655 and Bacillus subtilis 168 were used as models for Gram-negative and Gram-positive bacteria. The growth profiles and viability assays indicated that GO exhibited a high antibacterial activity, of which the negative effects on bacteria growth raised with the increasing GO concentration.
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PubMed
pubmed.ncbi.nlm.nih.gov › 37375132
Effects of Graphene Oxide Nanosheets in Freshwater Biofilms - PubMed
June 6, 2023 - A significant increase in the enzymatic (SOD and GSTs activity) and low molecular weight (lipids and carotenoids) antioxidant response was observed, that efficiently reduced oxidative stress effects, reducing the level of peroxidation, and preserving membrane integrity. Being complex entities, biofilms are more similar to environmental communities and may provide more accurate information to evaluate the impact of GO in aquatic systems. Keywords: antioxidant activity; freshwater biofilms; graphene oxide; oxidative damage; photosynthetic pigments; shading.
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Dovepress
dovepress.com › impaired-biofilm-development-on-graphene-oxide-metal-nanoparticle-comp-peer-reviewed-fulltext-article-NSA
Weakened Biofilm Formation on the Surface of Graphene Oxide-Metal Nan | NSA
December 24, 2024 - Nanocomposites based on graphene ... biofilms, contributing to changes throughout the biofilm structure, causing a significant reduction in the thickness of the structure, and affecting cell distribution....
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MDPI
mdpi.com › 2079-4991 › 12 › 3 › 355
Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms
January 22, 2022 - Indeed, P. aeruginosa formed biofilms which had on average 74% more biovolume and were 47% thicker than staphylococcal biofilms. Looking at the surface effect, the PDMS surfaces showed the highest biofilm amount and thickness (shadow projection on the right of Figure 6a,c) when compared to the graphene-based surface (Figure 6b,d).
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PubMed
pubmed.ncbi.nlm.nih.gov › 28003049
Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm - PubMed
February 15, 2017 - GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500mg/L. In contrast, rGO (≥50mg/L) strongly inhibited cell growth and biofilm formation.