Analysis of Stat3 (pS727) in monocytes.  Human peripheral blood mononuclear cells (PBMC) were either stimulated with 40 nM PMA (Sigma, P8139) for 15 minutes (shaded histogram) or unstimulated (open histogram).  The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD PhosFlow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with PE Mouse anti-Stat3 (pS727, Cat. No. 558557).  Monocytes were selected by scatter profile.  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Seiko Selection SBTM327 - men's watch manufactured by Seiko. It is equipped with Japanese solar quartz movement and 7B75 calibre. Titanium, hard coating case of round shape. Case dimensions are 39.5 mm. in diameter and 9.5 mm. in thickness. The dial of this Seiko watch is white. Display type: analog. This timepiece has sapphire glass. Titanium band, band color: silver. 100 m. water resistance. The watch is from Seiko Selection collection. The following features are equipped: world time, perpetual calendar, date, glowing hands, glowing markers, antimagnetic, radio controlled.