Co-cultures of E. coli TD2158 and S. enterica (ratio = 1:10) (A) or E. coli TD2158 and E. coli MG1655 (ratio 3:10) (B), were infected with the HK620::PrrnB-gfp phage and incubated at 30°C for 1 hour. The fluorescence intensity of individual cells gated to the bacterial population was determined ...
Tracking of E. coli TD2158 PL4 growth and division rates through phase-contrast microscopy.
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Structural studies of the O-antigen polysaccharide from Escherichia coli TD2158 having O18 serogroup specificity and aspects of its interaction with the tailspike endoglycosidase of the infecting bacteriophage HK620. (English)
April 1, 1998 - Only one registered well-developed phage plaques of which 2% were turbid-centered, typical of the temperate phage plaques. This strain was retained as TD2158 and will be the founder strain of the E. coli H lineage. Plating on TD2158 sewage samples, from the same stream but taken on different ...
In this study, we have constructed and characterized several "phagosensor" prototypes using the HK620 bacteriophage and its host Escherichia coli TD2158 and we successfully adapted this method to Salmonella detection. We show that the method is fast, robust and sensitive, allowing the detection ...
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We have analyzed the O-antigen polysaccharide of the previously uncharacterized Escherichia coli strain TD2158 which is a host of bacteriophage HK620. This bacteriophage recognizes and cleaves the polysaccharide with its tailspike protein (TSP). The polysaccharide preparation as well as ...
Phage HK620 was kindly provided by Mireille Ansaldi from University of Marseille and was propagated on E. coli H TD2158 as described elsewhere [21] and has O-serotype O18A1 [23]. E. coli strains were purchased from the German collection of microorganisms and cell cultures (DSMZ): DSM 10809 ...
Upper panels, bacterial growth and fluorescence profile obtained from non infected E. coli TD2158 alone (red), infected with WT HK620 (orange) or recombinant phages HK620::PbolA-gfp (purple), HK620::PrplU-gfp (green), HK620::PrrnB-gfp (blue) were compared. Top panels represent growth curves ...
HK620 displays a latent period of 40 min and a burst-size of approximately 400 PFU per infected TD2158 PL4 cell. Ciprofloxacin and cephalexin did not significantly modify the latent period, but produced an increased burst size of 28% and 36%, respectively.
Typhimurium DB7136 LT2 [37] was used, E. coli H TD2158 [38] served as false positive control. All other strains were from the stock culture collection of the National Reference Centre for Salmonellae and other Enterics at the Robert Koch Institute, Wernigerode, Germany, and are listed in Table 3.
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Zaccheus MV, Broeker NK, Lundborg M, Uetrecht C, Barbirz S, Widmalm G (2012) Structural studies of the O-antigen polysaccharide from Escherichia coli TD2158 having O18 serogroup specificity and aspects of its interaction with the tailspike endoglycosidase of the infecting bacteriophage HK620.
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